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Phenylgalactoside CAS:2818-58-8

Phenylgalactoside, quae p-nitrophenyl β-D-galactopyranoside (pNPG), synthetica subiecta est, saepe in experimentis biologiae biochemicis et hypotheticis adhibetur.Communiter adhibetur ad detectionem et modum actionis enzyme β-galactosidase.

Cum phenylgalactoside hydrolyzata ab β-galactosidase est, p-nitrophenolum emittit, quod mixtum luteum est.Liberatio p-nitrophenol utens spectrophotometris quantitatis mensurari potest, sicut absorbentia p-nitrophenol ad necem 405 um deprehendi potest.

 


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Application et effectus

Effectus in enzyme activitatem: Phenylgalactoside vulgo adhibetur ad metiendam actionem enzyme β-galactosidase.Cum phenylgalactoside hydrolyzata ab β-galactosidase est, p-nitrophenolum emittit.Cumulus p-nitrophenoli quantitatis mensurari potest, cum perspectionibus in activitate β-galactosidasi.Hic effectus in applicationibus alligatur ut pertentat enzyme et systemata protegendo.

Analysis gene expressionis: Phenylgalactoside saepe subiecta est experimentis biologiae hypotheticis ad studium gene expressionis.Gene lacZ, quod aba-galactosidase encodes, communiter est fusum cum consequentiis moderantibus aliorum generum usuris.Expressio gene lacZ et hydrolysis phenylgalactosi per β-galactosidas indicare potest expressio exemplaris et gradus scopo gene meditati.

Systema protegendi: Phenylgalactoside est magna pars systematum protegendi, qui β-galactosidasi actione utuntur.Exemplum late notum est methodus protegendi caeruleo-albo, quae ad cellulas recombinantes vel transformatas in experimentis biologiae hypotheticis cognoscendis adhibetur.Coloniae quae recombinationem DNA feliciter susceperunt vel recombinationem geneticam subierunt, β-galactosidase expriment, ducentes ad hydrolysin phenylgalactoside et caerulei coloris formationem.

Dapibus purificatio: In quibusdam casibus phenylgalactoside adhiberi potest ligando propter chromatographiam affinitatis ad purgandum servo, qui specialiter obligat vel operatur ab β-galactosidase.Dapibus usuris affinitatem habere potest tag vel fusione tag continens β-galactosidasi-constringens dominium.Transeundo interdum mixturam per columnam cum phenylgalactoside immobilitante, dapibus desideratus selective retineri et postea eludi potest.

 

Product sarcina:

6892-68-8-3

Additional Information:

Compositio C12H16O6
Assay 99%
Aspectus albumpulvis
CAS Non. 2818-58-8
stipare Parvus et mole
Fasciae vita Annis II
Repono Condite in frigus et siccum area
Certification ISO.

 


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